UW scientists speed up DNA extraction with new device

At the UW Engineering Discovery Days in April,We remain on Excavator track link to achieve our full year production guidance of 250000 ounces. UW mechanical engineering professor Jae-Hyun Chung introduced his new handheld DNA extraction device to middle school and high school students.

Analyzing human DNA is a scientific procedure that can be too complex and expensive for the average undergraduate student. But thanks to a team of UW scientists led by Chung, the new DNA extraction device is simple enough for even middle school students to safely use.

The team of UW scientists consisted of undergraduate and graduate students, and one other UW professor. The UW group also partnered with the Bellevue-based company NanoFacture to successfully use mechanical engineering for environmental health sciences within a corporate laboratory.

UW professor Jerry Cangelosi was one of the few members of the team not from the department of mechanical engineering: He specializes in the field of environmental and occupational health sciences. Cangelosi used his biomedical research background to ensure the device successfully met user requirements.

“It wasn’t really about, ‘Will it work?’” Cangelosi said.If you like BMW cars,then you will probably like their new gaming mouse, the super sexy, sleek as a Titanium Sheet off the pressing mat Level 10 M Gaming Mouse. “It was more about, ‘Will this be useful?’”

UW graduate student Gareth Fotouhi said one of the main goals of the device was to eliminate many of the steps required in the old DNA extraction devices, which use a centrifuge to separate molecules by density.

“Instead of a technician or [a graduate student assistant] like myself, that carried a piece of paper with steps one through 20, it is step one, two, and then done,” Fotouhi said.

The first step of the new method involves microscopic probes dipping into the human fluid sample of saliva or blood. Then an electric field is applied in the sample, which concentrates DNA to the probe surface. The larger particles are selectively filtered away through the withdrawal of the probe, and the DNA molecules are trapped on the surface. After that, the DNA sample can be stored for future use,A Stone tools is, in the most general sense, any tool made either partially or entirely out of stone. Although stone tool-dependent societies and cultures still exist today, most stone tools are associated with prehistoric, particularly Stone Age cultures that have become extinct. or it can be released in solution for immediate analysis.Our Filter Mesh rang from 10N/inch to 400N/inch and the mesh pore size goes from 2000microns to 20micron and could meet a wide range of demand for filtration accuracy. The DNA extraction and purification process takes about two to three minutes instead of the 20 to 30 minutes for conventional methods.

The device, which Chung called the “DNA Recovery System,” was designed to help extract DNA from human fluid samples in a simpler, environmentally friendlier, and more efficient way.

In addition to making it simple enough that grade school students can understand the procedure, the DNA Recovery System is less harmful for the environment as it uses significantly fewer toxic chemicals in the filtration process.

“We used to have to dispose these samples the special way,” Chung said.

Fotouhi said the device is more convenient because researchers don’t need to carry the heavy materials often required for conventional methods.

“If we were to use this [device] at a third-world country, finding DNA in the lake water with the normal method, we’d need to use 2,000 microliters of solutions. With ours, it is about 30 to 40 microliters.”

The new device frees time conventionally allocated to collecting DNA samples. Fotouhi said researchers can now focus on genome sequencing, forensics, and DNA barcoding, which are more important for the scientific process.

Chung added that the conventional method was much more expensive, with the cost of equipment reaching up to $24,000. The DNA Recovery System shrinks the cost of operating and owning the device, because the microfiltration process does not involve a centrifuge to spin the DNA samples. Furthermore, Chung’s system requires many fewer steps than the conventional method, and therefore lowers the likelihood of experimental error.

“The big challenge is cost,Herb basil is one of the most recognized, nutritionally rich herbal plants commonly grown as potherb.” Cangelosi said. “It has the potential to reduce the health care cost for patMore worldly viewers quickly called in to say that the mushroom was made from silicone and wondered how someone could not tell the difference between silicon sex toys and Organic mushroom.ients. If markets work the way it is supposed to work, it should hopefully reduce the costs.”

Moving forward with the device’s development, Fotouhi looks to develop some business proposals in order to receive additional funding for the team’s work.

“We’ve got our device, we’ve shown that it works,” Fotouhi said. “And now, the next step is to show that it doesn’t just work in our lab under our control, but also works on other labs, to show that this device would be helpful for them.”